Case Study by Dr Devicharan Shetty (DC Shetty) on Reproducibility


With the time, the advancements in the various fields is increasing day by day. Mainly in the medical field there are so many researches that are being done and are carried out as medical field directly involves the lives of all the living beings so it is one of the, most field of study. All these researches are being successful due to our some of the most talented researchers. One of the researcher about whom I am going to talk about is Dr Devi Charan Shetty. His work towards the medical industry is commendable and today I am going to tell you all about one of his research work.

One of the research work done by the Dr Shetty that is very mush valuable for all of us is Study on the Reproducibility of grading system in Oral Epithelial Dysplasia. Dr DC Shetty with his fellow Adesh Manchanada did research work in this field. The objective of the study was to assess the inter as well as intra observer variability in the grading oral epithelial dysplasia (OED) by making use of the Smith and Pindborg grading System. This is related to the cancer which has become one of the hazardous disease for all of us. Also for every diagnostically active pathologists grading of the dysplasia is demanded almost on the regular basis. The evaluation of the grading of the oral epithelial dysplasia is one of the greatest problems as it is mainly based on the subjective impression.

Research Outcomes on OED by Dr DC Shetty

The oral epithelial dysplastic lesion is also known as the pre cancer lesion and mostly occurs in the head and neck pathology. As stated by the Dr DC Shetty the concept of the stepwise development of the cancer in oral mucosa is very well established. Also presence of the epithelial dysplasis is very important in predicting the malignant development than clinical characteristics. Also according to the study of both the researchers a collaborating reference centre was also established by the WHO in 1967 with the objective to define as well as characterize those type of leisons which should be considered as the oral precancer and also to determine the relative risk of becoming the malignant. WHO published the “histopathological typing of the cancer and precancer of oral mucosa in the year 1997”.

Also the organisation divided the epithelial dysplasia as the Mild, Moderate and Severe.

Also according to the Dr Shetty, due to discrepancy of epithelial maturation patterns in assessing diagnosis of epithelial dysplasia is subjective. The right grading of the OED gives a big challenge to all the pathologist. Basically if we talk about the grading then it is the attempt to impose the discrete the categorization. So any of the grading effort is thus by definition artificial. The pathologists need to provide the information that is useful to the clinicians and also this is done by creating the discrete sub entities in the biological continuum. Till now the research work done by everyone, it is founded that unless the clear criteria are decided upon, it will always remain the debatable subject. So this is one of the most important of Dr Devicharan Shetty for the nation and for the medical industry and still more research is being carried out by him and his team.  

Research work on Amelogenin by Dr Devicharan Shetty (DC Shetty)

You all must have heard about many researchers and must also have read about their research work. So today I am going to tell you all about one of the researcher who is best in his field and is contributing towards the society with his studies. WI am talking about Dr Devicharan Shetty who is a well known researcher in the field of medical science. All of his research work till now is commendable. Today I am going tell you about one of his research work which everyone need to know.

The main background of the study that was done by Dr DC Shetty is to identify the sex of deceased individual from the dental hard tissue like dentine and enamel. The objective of this study was to isolate the DNA from the enamel and dentin from the teeth extracted for the prophylactic purpose so that to assess quality and purity of the DNA and also to indentify the sex using polymerized chain reactor (PCR).

Case Study on Identification of DNA by Dr Devicharan Shetty (DC Shetty)

As per the research work done by the Dr Shetty, the recognition of the importance of the human teeth in the personal identification have been recognized from time immemorial. PCR provides one of the efficient as well as the sensitive method of the sex determination by amplifying the gender specific sequence. For this a cross sectional study was carried out on total 40 samples from which 20 were male and 20 were females, from the teeth that was extraxted for the therapeutic purpose. Proper permission was taken from the institutional ethical committee (IEC) before starting the study.

The study was basically conducted to evaluate and also to establish the teeth and mainly the dental hard tissue as an important source of the DNA. Dr DC Shetty carried out this study for the extraction of the NA from the teeth samples that yields the sufficient amount of good quality of the DNA useful for the DNA for the PCR based diagnostic methods.  

The result of the study carried out was very helpful for the medical industry to work on this further. Dr DC Shetty used to work on the medical field and he giving us all so many new findings and we all are so overwhelmed with his work till now for the society. It is also said that he has dedicated all his life for the society and keep the research work going on. The study on the Amelogenin was very different kind of study which also includes PCR amplication in the study. For the PCR amplification the basic steps that re involved in this are Denaturation, Annealing and the last one is Extension. These 3 steps basically constitutes to the single cycle of the reaction. The research work was successful in isolating the DNA from the enamel and dentin as discussed above in the objective. Similarly like this study Dr DC Shetty have done research on various topics that are the important issue for the medical industry and with his research work and knowledge the industry is able to find the solutions of some of the major problems. So we all are thankful to Dr Devicharan Shetty for his valuable work towards the society.